山梨医科大学雑誌　第12巻4号　143-150(1997)

Immunization with Rat Thyrotropin Receptor Peptides
Corresponding to Two Specific Extracellular Domains Alters
Thyroid Function in vivo

Emiko OHTA, Toyoshi ENDO, Masayuki 0HMORl, Makoto 0HNO,
and Toshimasa ONAYA

Abstract: The relationship between the specificities of rat thyrotropin receptor antibodies and their biological effects on the rat thyroid in vivo was studied. Sixteen male Wistar rats were immunized with one of two synthetic peptides; one corresponding to the N-terminal region, amino acid residues 29-57, termed RAT-N, and the other corresponding to the C-terminal region of the extracellular domain, amino acid residues 341-370, termed RAT-P. Rats immunized with RAT-N possessed thyroidstimulating antibody (TSAb) activity and significantly higher serum concentrations of 3, 5, 3'-tri-iodothyronine (T3) than those of the control groups. In contrast, antibodies generated in rats immunized with RAT-P posscssed TSH-binding inhibitor immunoglobulin (TBII) activity and lower T3, levels than those in the control group. Histologically, there were no inflammatory or destructive reactions in the thyroid glands of any of the rats. These findings suggest that thyrotropin receptor antibodies against the N-terminus have stimulatory effects on thyrocytes, and that antibodies generated against the C-terminal region of the extracellular domain play an inhibitory role in thyroid function in vivo.

Key words: thyrotropin receptor, autoantibody, hyperthyroidism, hypothyroidism




本文は、編集委員会の意向によりインターネットには公開しておりません。図書館等でご覧ください。

Texts are not availavle on Internet.



目次・Contentsに戻る