山梨医科大学雑誌　第3巻3号　089-096(1988)

Immunocytochemical Localization of Serine : Pyruvate
Aminotransferase in Peroxisomes of the Monkey Liver

Sadaki Yokota, Toshiaki Oda, and Arata Ichiyama

Abstract: Intracellular distribution of serine: pyruvate aminotransferase (SPT) in liver cells of monkeys, Macaca fuscata was investigated using immunocytochemical techniques. Small tissue blocks of liver were immersion-fixed and embedded in Epon or Lowicryl K4M. For light microscopy (LM), after removal of the epoxy resin, semi-thin sections were stained for SPT by immunoenzyme techniques. For electron microscopy (EM), ultra-thin sections of Lowicryl K4M-embedded materials were stained by immunogold technique. By LM, a discrete granular reaction product for SPT was present in the cytoplasm of parenchymal cells. The staining pattern was consistent with that for catalase (peroxisomal marker), but different from that for ornithine transcarbamylase (mitochondrial marker). By EM, most of the gold particles indicating SPT were confined to peroxisomes, while mitochondria were mostly negative. In control sections incubated with absorbed antiserum, no gold label was noted. The results indicate that in monkey liver, SPT is associated with peroxisomes, but not with mitochondria.

Key words: Immunocytochemistry, Serine: Pyruvate Aminotransferase, Monkey Liver, Peroxisomes.




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