山梨医科大学雑誌　第3巻3号　097-103(1988)

Study on Lymphokine Activated Killer(LAK) Cells
I. Improved LAK cell induction in vitro

Hidehiko Iizuka,Hirofumi Naganuma,Noboru Yabusaki,
Hideki Komatsu, Toshiko Sakihama, Yuji Iimuro,
Yasuyoshi Yamamoto, Hideki Fujii, Masayuki Yamamoto,
Yoshiroh Matsumoto, Katsuhiko Sugahara, Hideaki Nukui,
Akira Ueno, Yasuo Nakajima, and Kachio Tasaka

Abstract: The optimum incubation period, serum or plasma concentration, recombinant interleukin 2(rIL-2) concentration, cell density and kind of culture bottles for inducing lymphokine activated killer (LAK) cells in vitro from normal peripheral blood lymphocytes (PBL) were examined. Culture of PBL, at a high cell density of 2×10^6/ml with 9 U/ml of rIL-2(TGP-3) and 2% of serum (or plasma) for 3 days in a flat bottle, was most effective, although the cell density of 1×10^6/ml and 3 U/ml of rIL-2 has been used so far. ABO matched allogeneic frozen plasma was shown to be as effective to induce LAK cells as AB sera. This Inodification could reduce the volume of medium, number of culture bottles and CO2 incubator space, and cost required in mass culture for practical use.

Key words: LAK, IL-2, Adoptive immunotherapy




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