山梨医科大学雑誌　第4巻4号　173-178(1989)

In vitro Production of Tumor Necrosis Factor (TNFα/Cachectin)
from Human Peripheral Blood Monocytes Stimulated
with Bacillus Calmette-Guerin. - A Possible
Mechanism of BCG Therapy

Noboru YABUSAKI, Hideki KOMATSU, Akira UENO,
and Kachio TASAKA

Abstract: Healthy human peripheral mononuclear cells cultured with bacillus Calmette-Guerin (BCG) alone produced cytotoxic factor that lysed L.P3 murine fibroblast cells after 2 hours of culture, and the production reached a peak after 24 to 48 hours. Since L.P3 cells are sensitive both to tumor necrosis factor (TNFα) and lymphotoxin (TNFβ), specific antibodies were used to identify this cytotoxic factor as TNFα, not lymphotoxin. This production of TNFα was shown to be derived from monocytes, because it disappeared after monocytes were depleted from mononuclear cells. Tumor necrosis factor induced with BCG may be a factor in the anti-tumor effect of topical therapy with BCG.

Key words: tumor necrosis factor, Bacillus Calmette-Guerin, human monocytes




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