山梨医科大学雑誌　第4巻4号　185-193(1989)

NMR Spectroscopic Studies on the Urine of Adult Male Mice

Tomoo NAKAZAWA, Takeru MINAMISAWA, Mariko ISHIHARA,
Kazuhiro MATSUSHITA, Hiroshi SUGIYAMA, and Masakazu YAMAGUCHI

Abstract: To develop a simple and non-invasive method for monitoring the metabolic behavior of experimental animals, NMR spectroscopy was applied to the study of mouse urine constituents. Mice from which urine was obtained were the first male generation hybrid of C57BL/6×C3H at 6 months of age. 1H-NMR spectra at 270 MHz or 400 MHz and 13C-NMR spectra at 100 MHz of the urine (0.3-0.5 ml) were measured by JEOL spectrometers. From the spectra obtained, we have identified citrate and 10 nitrogenous excretion products as urine constituents, in which trimethylammonium and dimethylammonium ions and trimethylamine oxide were confirmed by NMR and mass spectroscopies. The concentrations and the relative ratios of the urine constituents were determined and may serve a criterion for evaluating the metabolic behavior and physiological condition of mice.

Key words: NMR spectroscopy, mouse urine, trimethylammonium ion, dimethylammonium ion, trimethylamine oxide.




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