山梨医科大学雑誌　第5巻2号　111-119(1990)

An Experimental Study on Allograft of Cryopreserved Articular
Cartilage

Hideaki SATO, Tetsuo HAGINO, Yuwao YOKOYAMA, and Noriya AKAMATSU

Abstract: Allografting is a very useful means of reconstructing cartilage. An attempt was made to transplant a very thin cryopreserved articular cartilage film with no subchondral bone into the recipient cartilage defect, with the aim of reducing the rejection process.
1. Articular cartilage preservation
The knee joints of adult rabbits were pretreated with various kinds of cryopreservatives and saline, then rapidly coofed to -80℃ and stored for 2 months. Pretreatment with 5% DMSO was useful because the columnar arrangement of the cartilage cells was maintained and a great number of typical cartilage cells were obsreved.
2. Transplantation of articular cartilage
A thin circular shaped cartilage film (3mm in diameter) was removed from the condylar surface of the patello-femoral joint and the weight bearing surface of the tibio-femoral joint which was preserved for 2 months with 5% DMSO. The thin film was transplanted into the cartilage defect. No apparent immunological rejection was revealed histologically. The suecess rate was 80% (32 out of 40 sites). Suecessful cartilage film allografting with no apparent rejection was obtained in the specimen fitted with no gap into the recipient cartilage bed and most frequently at the non-weight bearing cartilage surface.

Key words: Chondral allograft, Cryopreservation, Articular cartilage




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