山梨医科大学雑誌　第5巻3号　151-161(1990)

Physical and Pharmacological Regulation of Atrial Natriuretic Peptide
Release from the Isolated, Blood-Perfused Right Atrium of the Dog

Shigeru MOTOMURA, Masanori OHTA, Kenji OHYAMA, and Keitaro HASHIMOTO

Abstract: Physical and pharmacological interventions in atrial natriuretic peptide (ANP) release were investigated using the canine isolated, blood-perfused right atrium preparation. Plasma ANP concentration was measured by radioimmunoassay. ANP secretion rate was calculated from plasma ANP concentration in venous blood leaving the right atrium preparation and blood flow through the right coronary artery. When perfusion pressure was increased stdpwise from 50 to 200 mmHg, ANP secretion rate was increased. ANP secretion rate was correlated to perfusion pressure and blood flow. ANP secretion rate was increased by atrial muscle distension by a 15-g weight loading and also by atrial tachycardia paced at a rate of 200 beats/min. Acetylcholine (3 μg) injected into the right coronary artery increased ANP secretion rate, which was blocked by 10 μg of atropine. Increased ANP secretion rate by 0.3 μg of noradrenaline was affected by neither 100 μg of atenolol nor 10 μg of prazosin. The increase by 3 μg of phenylephrine was blocked by prazosin, but unaffected by atenolol. Isoprenaline (0.03 μg) increased the ANP secretion rate, which was blocked by atenolol, but not by prazosin. Increases in sinoatrial rate by noradrenaline and isoprenaline were abolished by atenolol, but not by prazosin. Phenylephrine did not increase the sinoatrial rate. These results suggest that 1) the perfusion pressure of the right atrium is closely related to ANP secretion as well as atrial muscle distension and 2) ANP secretion is stimulated via alpha- and beta-adrenoceptors as well as muscarinic receptors.

Key words: Atrial natriuretic peptide, Perfusion pressure; Blood flow, Muscarinic receptor, Alpha-adrenoceptor, Beta-adrenoceptor




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