山梨医科大学雑誌　第6巻1号　039-043(1991)

The Effect of Ageing on Cell Cycle Kinetics
in Mouse Spleen Lymphocytes

Hitoshi HOSHINO, Tatsuya TAKESHITA, Zentaro YAMAGATA, Sumio IIJIMA,
Akio ASAKA, and Makoto HIGURASHI

Abstract: We examined the influence of ageing on mouse spleen lymphocytes. Spleen lymphocytes from young and old mice were cultured, and cell cycle kinetics were analyzed by the method of sister chromatid differential staining (SCD). Proliferation index and mitotic index of spleen lymphocytes were significantly lower in old mice than in young mice at several culture incubation times. These findings show that age-related changes of cell cycle kinetics in spleen lymphocytes are consistent with those of in vitro studies using human lymphocytes. Thus, the findings of the present study strongly suggest that the present culture system using mouse spleen lymphocytes is useful as an alternative model for studying age-related changes.

Key words: Ageing, Lymphocytes, Sister chromatid exchange, Cell cycle kinetics, Spleen




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