山梨医科大学雑誌　第7巻4号　169-172(1992)

Identification of Microsatellite Repeat Polymorphisms in the Placental Alkaline Phosphatase Gene

Zentaro YAMAGATA, Kathy HANDA Moyra SMITH, and Akio ASAKA

Abstract: Examination of the published sequence of placental alkaline phosphatase (PLAP) gene revealed the presence of (CA)n repeats at position 542-565. We utilized sequence information to design primers to amplify a DNA fragment of about 112 bases by means of PCR. Use of this primer set led to the identification of PLAP polymorphisms with six alleles. We used the DNA from human hamster somatic cell hybrids to confirm that the polymorphism was dependent on the presence of human chromosome 2. Use of this primer set also led to the amplification of the DNA present in the intestinal alkaline phosphatase (IALP) gene, using the clone IAP8, which contains the IALP gene. The product derived from the IALP gene migrated differently to the polymorphic alleles, in denaturing acrylamide gels. This polymorphism will be useful for linkage analysis.

Key words: Microsatellite repeat, Polymorphism, Polymerase chain reaction, Placental alkaline phosphatase, Chromosome 2




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