山梨医科大学雑誌　第8巻3号　121-124(1993)

Determination of TF Subtypes in Human Organ Tissues Using
Isoelectric Focusing and Immunoblotting

Akira KIDO, Yukiko KIMURA, Toshiko NISHIZONO, Toshio INOUE,
and Masakazu 0YA

Abstract: Transferrin (TF) subtyping in various human organ tissues (spleen, pancreas, heart,liver, muscle, lung, kidney, skin, brain, prostate, testis, ovary and uterus) was carried out using isoelectric focusing and immunoblotting. TF subtypes were demonstrated in fresh tissue samples of almost all the organs examined except a few samples of the pancreas and skin. In tissue samples of the heart, muscle, lung, kidney, brain, prostate, testis, ovary and uterus that were stored at room temperature, TF subtyping was possible for up to 1 week. The findings showed that the TF subtype system is useful for medicolegal individualization of human tissues.

Key words: TF subtyping, Isoelectric focusing, Immunoblotting, Individualization, Human tissues




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