山梨医科大学紀要　第11巻，021-023(1994)

Application of Crystal Violet Staining Assay to Drug
Sensitivity Test for Human Malignant Tumor

Eiichi SATO, Yoshiki HAMADA, Hideki KONO, Hiroyuki MASUYAMA,
Akichika YOSHIDA, Shingo MAEKAWA, Noriya AKAMATSU

We applied an assay using crystal violet to test for anticancer drulg sensitivity. In brief, after short-term incubation in a microplate, surviving tumor cells were fixed with methanol and stained with crystal violet. After washing out the dead cells, the remaining cells attached to the plastic plate were dissolved in ethanol and the intensity of absorbance was measured at 570 nm by spectro-photometer.
We investigated the relationship between the number of tumor cells and the intensity of absorbance and found that the intensity appeared to be in direct proportion to the number of surviving cells.
The reliability of the crystal violet assay was confirmed by the results of a 3H-thymidine uptake assays.
Our study demonstrated that the crystal violet assay is a simple, rapid and reliable method for-evaluation regarding anticancer drug sensitivity.
key words: Drug sensitivity testing, Crystal violet assay, Human tumor cell lines, 3H-thymidine uptake assays




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